解剖学报

›› 2010, Vol. 41 ›› Issue (4): 532-537.doi: 10.3969/j.issn.0529-1356.2010.04.010

• 论著 • 上一篇    下一篇

Bmi-1基因对人胚胎纹状体来源神经干细胞的自我更新和增殖的作用

黄爱华1, 3; 梁庆成1*; 吉慧军2 ;王旸3 ;关云谦3    

  1. 1.哈尔滨医科大学附属第二临床医学院神经内科， 哈尔滨 150086;2.青岛大学附属医院神经外科， 青岛 266003;3.首都医科大学宣武医院细胞治疗中心，北京 100053
  • 收稿日期:2009-10-09 修回日期:2009-12-28 出版日期:2010-08-06
  • 通讯作者: 梁庆成

Role of Bmi-1 in the self-renewal and proliferation of neural stem cells isolated from human corpus striatum

  1. 1. Department of Neurology, the Second Clinical Hospital of Harbin Medical University,Harbin 150086,China;2. Department of Neurosurgery, the Hospital Affiated to Medical College,Qingdao University, Qingdao 266003, China;3. Cell Therapy Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
  • Received:2009-10-09 Revised:2009-12-28 Online:2010-08-06
  • Contact: LIANG Qing-cheng

关键词: 神经干细胞, RNA干扰, 更新, 增殖, 实时定量聚合酶链反, 人

Abstract: Objective To discuss whether Bmi-1 is participated in maintenance of the self-renewal and proliferation of neural stem cells (NSCs) isolated from fetal striatum. Methods Neural stem cells were transfected with Bmi-1 targeted shRNA-lentiviral vectors and GFP control vector respectively. At 0 hour,48 hours, 72 hours, and 168 hours after transfection, the Bmi-1 mRNA expression level was measured by Realtime PCR. The numbers and diameters of newly formed neurosphere were calculated under microscope. Results The transfection efficiency was shown to be more than 90% by expression of reporter gene GFP. At 0 hour,48 hours, 72 hours and 168 hours after transfection, transcription level of Bmi-1 were to be （107.3±8.0618）%, （32.7±9.74）%, （24.4±12.15）%, （32.7±10.39）% compared with 0 hour, the relative expression levels of Bmi-1 at 48 hours, 72 hours, and 168 hours were significantly decreased(EM>P/EM><0.05). Accordingly, The number and size of newly formed neurospheres were als

Key words: Neural stem cell, RNAi, Self-renewal, Proliferation, Real-time PCR, Human

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